Electron microscopy of typical CD11b^−/dullCD11c⁺ precursor and its derived CD11b^−/dullCD11c⁺ mature DCs. The CD11b^−/dullCD11c⁺ precursors and its derived CD11b^−/dullCD11c⁺ mature DCs were sorted at culture day 6 and day 12, respectively, and processed for electron microscopy staining as described in Materials and Methods. (A) A representative of CD11b^−/dullCD11c⁺precursor sorted at day 6. Original magnification ×5,300. (B) A representative of CD11b^−/dullCD11c⁺precursor-derived CD11b^−/dullCD11c⁺ mature DC sorted at day 12. Original magnification ×5,300. (C) A representative of CD11b^+hiCD11c⁺ precursor sorted at day 6. Original magnification ×9,600. (D) A representative of CD11b^+hiCD11c⁺ precursor-derived mature DC with the phenotype of CD11b^−/dullCD11c⁺sorted at day 12. Original magnification ×9,600.