The day 6 DC precursors differentiate into cells with typical DC markers. CD11b^−/dullCD11c⁺and CD11b^+hiCD11c⁺ precursors were sorted from murine Lin⁻c-kit⁺ HPC cultures stimulated with GM-CSF + SCF + TNF-α for 6 days and were cultured in the presence of GMCSF + TNF-α for an additional 5 to 8 days. At days 12 to 14, the phenotype of the CD11b^−/dullCD11c⁺ DC-like cells derived from either CD11b^−/dullCD11c⁺ or CD11b^+hiCD11c⁺ precursors was determined using three-color analyses as described in the legend to Fig 2.