American Society of Hematology

Fig. 7.

$Fig. 7. The capacity of the cultured cells to enhance allogenic MLR. Allogenic MLR was performed using purified T cells (3 × 105 cells per well in 96 round-well plates) as responder cells. (A) The day 6 sorted CD11b−/dullCD11c+, CD11b+hiCD11c+, and CD11b−CD11c− precursors, and unfractionated cells from Lin−c-kit+ HPC cultures were treated with MMC and used as stimulators at the indicated cell number. (B) Sorted mature CD11b−/dullCD11c+ mature DCs derived from CD11b−/dullCD11c+, CD11b+hiCD11c+ DC precursors at days 12 to 14 and macrophages derived from M-CSF–induced CD11b+hiCD11c+ DC precursors were used as stimulator cells at the indicated cell number. The proliferation of T cells was measured by MTT assay after 4 days of culture. Results are expressed as mean ± 1 SD of triplicate cultures. Results of each panel are representative of three independent experiments.$

The capacity of the cultured cells to enhance allogenic MLR. Allogenic MLR was performed using purified T cells (3 × 10⁵ cells per well in 96 round-well plates) as responder cells. (A) The day 6 sorted CD11b^−/dullCD11c⁺, CD11b^+hiCD11c⁺, and CD11b⁻CD11c⁻ precursors, and unfractionated cells from Lin⁻c-kit⁺ HPC cultures were treated with MMC and used as stimulators at the indicated cell number. (B) Sorted mature CD11b^−/dullCD11c⁺ mature DCs derived from CD11b^−/dullCD11c⁺, CD11b^+hiCD11c⁺ DC precursors at days 12 to 14 and macrophages derived from M-CSF–induced CD11b^+hiCD11c⁺ DC precursors were used as stimulator cells at the indicated cell number. The proliferation of T cells was measured by MTT assay after 4 days of culture. Results are expressed as mean ± 1 SD of triplicate cultures. Results of each panel are representative of three independent experiments.

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