Western blot of normal and deleted A subunits in fresh lysates of S cerevisiae AH22. Total yeast lysate protein was prepared from S cerevisiae transformed with lane 1, pGal181/wild-type factor XIII A-subunit cDNA (1.5 μg); lane 2, negative control vector pGal181 (1.5 μg); and lane 3, pGal181/Asn 344–deleted factor XIII A-subunit cDNA (6 μg). The samples were subjected to SDS-PAGE, blotted onto nitrocellulose membrane, and developed with antiserum to human factor XIII A subunit.