RNA hybridization analysis of factor XIII A-subunit expression in yeast. (A) Ethidium bromide stained gel; (B) hybridized membrane. Total RNA was prepared from S cerevisiae AH22 transformed with negative control vector pGal181 (lanes 1, 4), pGal181/wild-type factor XIII A-subunit cDNA (lanes 2, 5), or pGal181/Asn344–deleted factor XIII A-subunit cDNA (lanes 3 through 6) and grown either in YPGal (induced; lanes 1 through 3) or YPD (repressed; lanes 4 through 6). Hybridization with a factor XIII A-subunit probe and chemiluminescent detection were performed as described in Materials and Methods. RNA size standards (Promega) on the left are in nucleotides.