Northern blot analysis of the cDNA clone 2.1. OTT-1 cells were deprived of serum and mIL-3 for 6 hours, and then stimulated with mIL-3 and mGM-CSF for 1 hour. Poly(A)+ RNA (4 μg/lane) was hybridized with the cDNA (clone 2.1) and G3PDH probes.
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