Fig. 2.
![Fig. 2. Pulse-chase analysis of metabolically labeled early erythroblasts. Early erythroblasts were labeled with [35S]methionine for 60 minutes and then chased with unlabeled methionine for 48 hours. Plasma membranes isolated from unchased and chased cells were extracted with C12E8. (A) The C12E8extracts were subjected to size exclusion HPLC. (B) C12E8-insoluble pellets (P) and -soluble supernatants (S) were immunoprecipitated with anti–band 3 antibodies. The immunoprecipitates were analyzed by SDS-PAGE and the gels were processed for fluorography. Band 3 tetramers synthesized before the chase were stable and did not convert into dimers during the 48-hour chase period.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.329.413k20_329_338/5/blod41320002w.jpeg?Expires=1736046506&Signature=I96U4icjm7jQtT4Si8Twr4nHDx3Hu3UqKHhBtvyBWPEzm0i0gYdjxsxx9-6MqLAzN4ZdaKYNEfFg4h~joSV80SRM400~Wu~WxKsbIHc4KpAgtUXxSYv7ZDCgUB9RKk65OUtFKO46r9IGKAYGyyab7i1SFbuIrAWF~EouDUKaCP~6oBtp4DvX9gXPgQn3HjugVIcxWkYJr25NjcgAH5h3xTN6q1WOIixdx3jkKv4X30gvebSGTsxJeEfgCAfKSROyhLnWx~2rQEgLlWfrncpLSoyDyx3mfh6y~LA~yfBO9rCaqHJppOcJXvtg4f6RHOKKY3abz4~SCq96RWvFpEM6sg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Pulse-chase analysis of metabolically labeled early erythroblasts. Early erythroblasts were labeled with [35S]methionine for 60 minutes and then chased with unlabeled methionine for 48 hours. Plasma membranes isolated from unchased and chased cells were extracted with C12E8. (A) The C12E8extracts were subjected to size exclusion HPLC. (B) C12E8-insoluble pellets (P) and -soluble supernatants (S) were immunoprecipitated with anti–band 3 antibodies. The immunoprecipitates were analyzed by SDS-PAGE and the gels were processed for fluorography. Band 3 tetramers synthesized before the chase were stable and did not convert into dimers during the 48-hour chase period.
