Fig. 4.
![Fig. 4. Turnover of band 3 in the microsomal and plasma membrane fractions of early and late erythroblasts. (A) Microsomes and plasma membranes were isolated from erythroblasts labeled with [35S]methionine for 30 minutes and chased for different time periods (0 to 120 minutes). Band 3 was immunoprecipitated using anti–band 3 antibodies. The immunoprecipitates were analyzed by SDS-PAGE and the gels were processed for fluorography. B3: Band 3. (B) The autoradiograms shown in (A) were scanned and the area under each peak integrated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.329.413k20_329_338/5/blod41320004w.jpeg?Expires=1735811249&Signature=ut6G2oqmEy8YJjNDOxmoQl~0IDsIzNT~RZW8DjEzVBLMmXVKbG8LFdaeIBoYd6zjbLQSMvMjStO~B2PwNNAY5dXmdKaCApn~~0vpv0lx~Ens7ui01LOswgz5bk7H57incJFBM3dYg5iMATCzd1ZGuAXWcQXHPW5sfaA6~xjZkOGDh9TKWjeWlcFlUVMyvG9Hf~LaXGuUeW9RXpEPD1W7iA1tQSWnjggedbXXZsu0A5lSFaDIEnMROaY2VEFbQIn4-DJrpwaKs7oMd8R~j8hRZV3QJk~P4PT4luFMKZ4hd5iYaKQBhIdpyuV1zntlPI7MyW2HiyugfsiIuO8h92eCeA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Turnover of band 3 in the microsomal and plasma membrane fractions of early and late erythroblasts. (A) Microsomes and plasma membranes were isolated from erythroblasts labeled with [35S]methionine for 30 minutes and chased for different time periods (0 to 120 minutes). Band 3 was immunoprecipitated using anti–band 3 antibodies. The immunoprecipitates were analyzed by SDS-PAGE and the gels were processed for fluorography. B3: Band 3. (B) The autoradiograms shown in (A) were scanned and the area under each peak integrated.
