Fig. 5.
![Fig. 5. Endo H digestion of immunoprecipitated band 3 from early and late erythroblasts. Early and late erythroblasts were either [35S]methionine-labeled for 30 minutes (lanes 1, 2, 5, and 6) or labeled for 30 minutes and chased for 4 hours (lanes 3, 4, 7, and 8). NP-40 extracts were immunoprecipitated with band 3 antibodies, and half of the samples were incubated with (lanes 2, 4, 6,and 8) or without (lanes 1, 3, 5, and 7) Endo H before the separation of proteins on an SDS gel.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.329.413k20_329_338/5/blod41320005w.jpeg?Expires=1735811615&Signature=PYfB6pcTcs3k6l4BMC1I9952fetbSjBiXvEnreNTUqRa-KNo5WyJg6SPSqM-VufC~V03lscs~~38dQ-HxU0El-b0WWa0x5asgyYh3xYvpbmlTnkWxub3iv8Mugx5qF3ZpEvlImNGcOmtuEbx~YREBdtcrB9v3ujFRO~nHgAcnZG03KUIF65W2w5hpGA86QkeoEEINSIi7iZQu0vlagymO54FoXCG6wBIPREfz~f0S1GoyAXGo42WcamU6PGarcnXbo~9YHlk-tYYCjfFLfpdusC6FAxqm9qJ2fzZaBgI3wdeN5PeOghlIV-NnY0VWIbTFzrmsj3Iolf7BjxKb0ob7Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Endo H digestion of immunoprecipitated band 3 from early and late erythroblasts. Early and late erythroblasts were either [35S]methionine-labeled for 30 minutes (lanes 1, 2, 5, and 6) or labeled for 30 minutes and chased for 4 hours (lanes 3, 4, 7, and 8). NP-40 extracts were immunoprecipitated with band 3 antibodies, and half of the samples were incubated with (lanes 2, 4, 6,and 8) or without (lanes 1, 3, 5, and 7) Endo H before the separation of proteins on an SDS gel.
