IL-6 protects against anti-fas and dexamethasone. 8226 (lanes A through F) and UCLA #1 cells (lanes G through K) were cultured in media alone for 48 hours (lanes A and G), IL-6 alone for 48 hours (1,000 U/mL, lane B), anti-fas for 20 hours (0.5 μg/mL, lanes C and H), dexamethasone for 48 hours (10⁻⁶ mol/L, lanes E and J) or the combination of anti-fas + IL-6 (lanes D and I) or dexamethasone + IL-6 (lanes F and K). In the anti-fas + IL-6 combination, IL-6 was present for 1 hour before addition of anti-fas. DNA was then extracted and electrophoresed. Culture of both cell lines with a control antibody of identical isotype to the anti-fas antibody resulted in normal, intact, high-molecular-weight DNA (identical to that shown in lanes A and G [not shown]).