(A) Dexamethasone and anti-fas activate and IL-6 inhibits jun kinase activity in UCLA #1 cells. Top band: UCLA #1 cells were incubated in dexamethasone alone (lane A, 10−6 mol/L, for 10 minutes), anti-fas alone (lane B, 0.5 μg/mL for 10 minutess), media alone (control, lane C), dexamethasone (10−6 mol/L) + IL-6 (lane D, IL-6 at 1,000 U/mL present for 60 minutes before addition of dexamethasone), anti-fas + IL-6 (lane E, IL-6 present for 10 minutes before anti-fas), or anti-fas + IL-6 (lane F, IL-6 present for 60 minutes before anti-fas). In vitro kinase assay performed for jun kinase activity as shown in Fig 6. Bottom band: UCLA #1 cells incubated in media (lane A), dexamethasone (10−6 mol/L, lane B), or anti-fas (0.5 μg/mL, lane C) for 10 minutes. Jun kinase then immunoprecipitated from protein lysates and tested against GST-jun in in vitro kinase assay. (B) Coomassie blue staining of gel shown in upper band of (A).
