Colony-forming assay of fetal livers and yolk sacs. (A) Colony-forming assay of 3 × 10⁴ fetal liver cells of Mll^+/+, Mll^+/mu, and Mll^mu/muembryos at E12.5. The number of colonies of Mll^+/+ (n = 13), Mll^+/mu (n = 26), and Mll^mu/mu (n = 7) after 7 days of culture is shown. (B) Colony-forming assay of 3 × 10⁴ fetal liver cells of Mll^+/+, Mll^+/mu, and Mll^mu/mu embryos at E12.5. The number of colonies of Mll^+/+ (n = 8), Mll^+/mu (n = 22), and Mll^mu/mu (n = 5) after 14 days of culture is shown. (C) Colony-forming assay of yolk sac of Mll^+/+, Mll^+/mu, and Mll^mu/mu embryos at E 10.5. The number of colonies of Mll^+/+ (n = 8), Mll^+/mu (n = 8), and Mll^mu/mu (n = 5) after 7 days of culture is shown. (D) Colony-forming assay of yolk sac of Mll^+/+, Mll^+/mu, and Mll^mu/mu embryos at E 10.5. The number of colonies of Mll^+/+ (n = 8), Mll^+/mu (n = 8), and Mll^mu/mu (n = 5) after 14 days of culture is shown. n, number of embryos analyzed. Bars indicate standard deviations, and (▪) indicates the average of the number of colonies. Statistically significant difference at #$<.001, ♠⧫+<.01, *<.05. Statistical analysis was performed by Student's t-test and Welch's t-test.