Flow cytometric cytokine expression on cord and adult lymphocytes. The method for cytokine analysis is described in Materials and Methods. The top two traces show the forward scatter/side scatter distribution and the gate used to select lymphocytes for analysis. The quadrants were set using isotype controls for each of the antibodies. Percentages indicated in the right upper quadrant indicate the percentage of the total lymphoid population (ie, the cells within the region [R1] outlined in the dual scatter plot). For each plot cord blood (left column) is compared with the adult blood (right column).