Effects of overexpression of Stat1α and/or Stat3 on the EPO-induced differentiation of UT-7/GM cells. (A) UT-7/GM clones transfected with vector alone (lane 1; n = 10), Stat1α-transfected clones (lane 2; n = 10), Stat3-transfected clones (lane 3; n = 10), and Stat1α and Stat3-cotransfected clones (lane 4; n = 3) were cultured with EPO (10 U/mL). Seven days later, cells were harvested for dianisidine-staining. The data are the mean ± SD. (B) Parent UT-7/GM cells, Stat1α-transfected clones (clones 29, 32, and 47), and Stat3-transfected clones (clones 5, 12, and 29) were cultured with EPO (10 U/mL) and harvested for the isolation of total cellular RNA. γ-Globin and ALAS-E transcripts were detected by Northern blotting. The membrane was rehybridized with a³²P-labeled human ribosomal DNA probe to show the amounts of RNA loaded.