HLA restriction of the CTL lines. Cytolysis of the T2 HLA-A2–deficient line and HLA-A-1/3; -B7/8 EBV-transformed target cells unpulsed or pulsed with AML peptides eluted from the blasts of patient HAR was assayed. Representative results are shown for CTL 1 and 7 for an E:T ratio of 4:1. (▩), T2 HLA-A2 + AML HAR peptides; (□), T2 HLA-A2; (), EBV HLA-A1/3; B7/8 + AML HAR peptides; (▧), EBV HLA-A1/3; -B7/8.