Recognition of leukemic blasts of patient HAR. Secretion of TNF by clones was quantified (as described in Materials and Methods) after stimulation by the PBMC of the donor pulsed with AML peptides eluted from patient HAR () or patient LED (□) and after stimulation by leukemic blasts from patient HAR (▩) or patient LED (▨). Representative results are shown for the 7.1 and 7.2 clones.