Fig. 2.
Determination of transgene copy number and mosaicism in embryonic day 15.5 F0 transgenic mice containing the BGT33 construct. (A) Copy numbers of BGT33 transgenic mice determined by Southern blot analysis of F0 fetal head DNA digested withBamH1 and hybridized with the βivs2 probe. (B) Transgene mosaicism levels determined by Southern blot analysis of Acc1 digested DNA extracted from BGT33 fetal livers and hybridized with the βivs2 (Hβ) and mouse Thy-1 (mThy-1) probes. The percentage of transgenic cells was calculated by comparison of the Hβ band intensity of each BGT33 transgenic mouse with the one copy-bred line (B26), taking into account copy number and the mThy-1 loading control (see Materials and Methods).
