Human 5′HS1 core sequence and nine in vitro DNaseI footprints detected using MEL cell nuclear extracts. Footprints are boxed and labelled (A-C, D1-D3, and E-G). Consensus binding sites are underlined for Sp1 (footprint E), GATA-1 (footprints A, C, F, G), and the ACTAAC motif (footprints D1, D2). Hypersensitive bases are indicated by vertical arrows, horizontal arrows correspond to primers used for cloning 5′HS1 by PCR, dashed line represents the polylinker of the pGEM-T vector.