Fig. 6.
![Fig. 6. Hydrolysis of the prodrug N-[4-doxorubicin-N-carbonyl (-oxymethyl) phenyl] O-β-glucuronyl carbamate (100 μmol/L, closed symbols) to the cytotoxic drug doxorubicin (open symbols) by human β-glucuronidase (▵ and ▴, 1 μg/mL) or scFv-CD20–β-glucuronidase (○ and •, 1 μg/mL) as determined by reversed-phase HPLC and fluorescence detection.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.184.413k26_184_190/5/blod41326006x.jpeg?Expires=1735533489&Signature=noj4UFvh0SXhdcU-uj4-9gW870gsc578pZ6UL5Ok5L3Cst6Ck1JEP2DIAolPRTsY8LMdaD6s1sa~RzNO-xb3vnkwZ13kTOMc9u46IBv2T1~dfUKsHjsHIxSJOL3YiK-POMWjKsxENUPgFwZdgB328l9DlS1hfmOCOTByl0yhTEaSAciEQFbjtGgHsZY3vgaStZIiPN-gLchOfpbCFk5d5GwxVXFABkw9kbMRpJAv2r7QqgbAvK5vas3iZIUb~MKmMYLCNqubMxpcq4SmHkvTFTYBu7WVixTl681JNamX0kuHkRvc-WJu8UMgHEzcbMVCa4dKkkWmmythks651XUtiA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Hydrolysis of the prodrug N-[4-doxorubicin-N-carbonyl (-oxymethyl) phenyl] O-β-glucuronyl carbamate (100 μmol/L, closed symbols) to the cytotoxic drug doxorubicin (open symbols) by human β-glucuronidase (▵ and ▴, 1 μg/mL) or scFv-CD20–β-glucuronidase (○ and •, 1 μg/mL) as determined by reversed-phase HPLC and fluorescence detection.
