Fig. 7.
![Fig. 7. Antiproliferative effects of doxorubicin, prodrug N-[4-doxorubicin-N-carbonyl(-oxymethyl) phenyl] O-β-glucuronyl carbamate (DOX-GA3), and prodrug combined with scFv-CD20–β-glucuronidase. Cells were incubated with fusion protein, blocking antibody, and fusion protein, or PBS. Cells were then seeded in 96-wells plates and exposed to drug or prodrug for 24 hours. After 72 hours, cell growth was measured with a protein dye, Sulforhodamine B.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/1/10.1182_blood.v92.1.184.413k26_184_190/5/blod41326007x.jpeg?Expires=1735534289&Signature=hHt8g5vyP4M2we-RkC5ItxyiaoxK69JAezcESZP3mOMY0mHBwPYLmiikg1kUSKFjG4OpnWs-sUtciJK0mp2ZyvV0Sa4hqgLs85SUp05D4owFfud5MagtzJlmSw1V9kCGNYF9vQsw8syZDlJcJxjHzGNPl~bpLKkQ2ZqZOfwisEDgFsBGlVeyoNWqSf3M1AkkV8rvaLCIdHjSwpEYu35xTOPy-n0vZ7HZcI28gLd5Bj0d7v3tldqrnw2xItRr8OAMcZq0oUkX5aXsKD6SlK6HM5fzsuDKs6ku2ahSDDDe1AF0DpriwsjV5RQLbfmBq4Oq9NZNR89jOfobEh4H9H~zbg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Antiproliferative effects of doxorubicin, prodrug N-[4-doxorubicin-N-carbonyl(-oxymethyl) phenyl] O-β-glucuronyl carbamate (DOX-GA3), and prodrug combined with scFv-CD20–β-glucuronidase. Cells were incubated with fusion protein, blocking antibody, and fusion protein, or PBS. Cells were then seeded in 96-wells plates and exposed to drug or prodrug for 24 hours. After 72 hours, cell growth was measured with a protein dye, Sulforhodamine B.
