BO2C11 binding to full-length rfVIII or recombinant fragments and domains. Microtitration plates coated with the indicated purified proteins or polypeptides were incubated with 5 μg/mL of BO2C11 for 2 hours at 4°C. The binding of BO2C11 was detected by addition of a HRP-labeled mouse MoAb specific for human Fcγ. GST-fVIII: 2125-2332 includes exon 23 and exons 24 to 26 forming the C2 domain.