BO2C11-dependent inhibition of the binding of fVIII to PS and vWF. To assess the capacity of BO2C11 to inhibit the binding of fVIII to PS (▪) or to vWF (•), rfVIII at 2 μg/mL or 0.2 μg/mL final concentration, respectively, was mixed for 30 minutes at 37°C with different concentrations of BO2C11 before addition to PS- or vWF-coated plates, as appropriate. In both cases, the plates were then incubated for 2 hours at RT and the binding of fVIII was detected by the addition of the anti-A2 MoAbF15B12.