Kinetics of fVIII/BO2C11 association. BO2C11 was bound to the sensor chip surface by covalent coupling. Real-time binding was measured for rfVIII at the concentrations indicated. Between each experiment, surface-bound rfVIII was washed off by incubation with HCl, pH 2.0, for 36 seconds. The SPR response gives the amount of surface-bound component at each stage of the reaction, namely (A and B) baseline resonance signal; (B and C) association phase; and (C and D) dissociation phase.