Transgene expression in CD2-p13 mice. Detergent lysates were subjected to SDS-PAGE through a 15% gel. Immunoblot analysis was performed using the anti-p13 rabbit antiserum (upper panel) and loading was estimated using anti-actin monoclonal antibody (lower panel). (A) Expression analysis in the three transgenic lines. Thymocytes (Thymus) and splenocytes (Spleen) were analyzed in transgenics (F3, F4, and F7) and nontransgenic control mouse (C). (B) Expression analysis in the lymphoid subpopulations. CD3⁺ cells (CD3), CD4⁺ cells (CD4), CD8⁺ cells (CD8), and B220⁺ B cells (B) from a F3 transgenic mouse spleen were purified by cell sorting (purity >95%) and compared with total splenocytes of this mouse (total). Arrows indicate the p13-and actin-specific signals.