Fig. 3.
Effect of MBP on 3H-5HT release in different populations of murine mast cells. BMCMC grown in stem cell factor (BMCMC in SCF), BMCMC grown in Concanavalin A stimulated spleen conditioned medium (BMCMC in CM), or the growth factor independent cloned mast cell line, Cl.MC/C57.1, was incubated with3H-5HT for 2 hours, washed and then challenged with different concentrations of MBP (A) or poly-L-arginine (B) for 10 minutes. Specific 5-HT release was calculated as described in Materials and Methods. Results are expressed as mean ± SD (n = 3). ***P < .001 by the two-tailed paired Student'st-test. Similar results were obtained in a repeat experiment.
