Expression of P-selectin in rat liver 3 to 6 hours after the administration of LPS. Endothelial cells of the large portal vein (PV) and its branchings showed intense reaction to anti–P-selectin Ab (arrows; a). The endothelia of sublobular veins (V) and central veins (CV) also expressed P-selectin (b). P-selectin was also present along the walls of sinusoids and veins. A strong Ab reaction in the endothelia of central veins, as well as the pericentral sinusoids, was observed (c). The Ab reaction was relatively weak in the endothelium of periportal sinusoids but strong in adherent platelets (arrows, d), which were labeled by MoAb CD61 to platelet (arrows; e). Bars, a, b, and d = 50 μm; c = 50 μm; e = 10 μm.