Localization of P-selectin in intralobular area of the liver 3 to 6 hours after the administration of LPS. Immunostaining was with anti–P-selectin Ab (blue) and counterstaining was by nuclear fast red (red). Many platelets expressing P-selectin were found in the periportal area, correlating closely with the presence of PMNs (arrows; a). P-selectin was expressed on the endothelial cells of portal veins (PV), but not on ones of the periportal arteries (A; arrow) in the periportal tract (b). Bars = 40 μm.