Caspase-mediated apoptotic cell death induced by resveratrol (A) SDS-PAGE analysis of caspase-specific PARP cleavage in lysates of resveratrol-treated HL60 cells. Tumor cells (2 × 10⁶) were treated with resveratrol (8 to 32 μmol/L) for 24 hours, lysed, subjected to 10% SDS-PAGE, and transferred to nitro-cellulose membranes as described in Materials and Methods. PARP cleavage was detected using a primary monoclonal antibody C-2-10 followed by the secondary HRP-conjugated anti-mouse IgG. Arrows indicate the intact protein of 116 kD and the proteolytic cleaved fragment (85 kD). (B) Inhibition of apoptotic cell death in HL60 cells treated with 32 μmol/L resveratrol for 24 hours in the presence of 2.5 μmol/L caspase inhibitors DEVD-CHO or YVAD-CHO or both. Cytotoxicity was determined by the MTT assay, and data shown are mean ±SD of three independent experiments.