Resveratrol enhances CD95L expression and induces CD95 signaling–dependent cell death in T47D cells. (A) T47D cells were treated with 32 μmol/L resveratrol for 18 hours, and surface expression of CD95 and CD95L was analyzed by flow cytometry as described in Materials and Methods. The shaded histograms are for mouse IgG1κ used as an isotype control, the dotted lines represent nontreated T47D cells, and the solid lines indicate resveratrol-treated T47D cells. At least 10,000 events were counted, and data shown are representative of at least three separate experiments. (B) T47D cells were treated for 24 hours with 32 μmol/L resveratrol alone (medium) or in the presence of 1 μg/mL of anti-CD95 (anti-CD95) or IgG1κ (isotype Ig) antibody. Cell viability was determined by the crystal violet assay, and data shown are mean ±SD of three independent experiments.
