Effect of resveratrol on normal human PBL survival and cell surface expression of CD95 and CD95L. (A) Normal human PBLs were treated with resveratrol (8 to 32 μmol/L) for 24 and 72 hours as described in Materials and Methods. Cell viability was determined by the MTT assay and represents three independent observations. (B) Flow cytometry analysis of CD95 and CD95L expression on normal human PBLs was performed as for HL60 cells, except that PBLs were analyzed for 12, 24, and 72 hours. The shaded histograms are for mouse IgG1κ used as an isotype control, the dotted lines represent nontreated PBLs, and the solid lines indicate resveratrol-treated PBLs. At least 10,000 events were counted, and data shown are representative of at least three separate experiments.
