Fig. 4.
Fig. 4. Abnormal development of Hoxa-9−/−thymocytes in 6-day FTOCs. (A) FACS analysis of thymocytes harvested from 6-day FTOCs using antibodies against CD4, CD8, and αβ TCR. There is a marked decrease in the number of DP cells (upper right quadrant of middle panels) in the mutant thymuses, associated with a 10-fold increase in the percentage of CD8+ cells (lower right quadrant). These latter cells are ISPs, ie, TCR−CD8+, as shown in the lower right side panels, where the numbers represent ISP cells as a percentage of total thymocytes. (B) Absolute numbers of T-cell subsets in 6-day FTOCs. Based on analyses from 4 separate FTOC experiments (representing 7 mutant and 8 wild-type thymuses), there is a delay in progression ofHoxa-9−/− thymocytes through the ISP stage and significant reductions in the numbers of more mature DP and SP cells.

Abnormal development of Hoxa-9−/−thymocytes in 6-day FTOCs. (A) FACS analysis of thymocytes harvested from 6-day FTOCs using antibodies against CD4, CD8, and αβ TCR. There is a marked decrease in the number of DP cells (upper right quadrant of middle panels) in the mutant thymuses, associated with a 10-fold increase in the percentage of CD8+ cells (lower right quadrant). These latter cells are ISPs, ie, TCR−CD8+, as shown in the lower right side panels, where the numbers represent ISP cells as a percentage of total thymocytes. (B) Absolute numbers of T-cell subsets in 6-day FTOCs. Based on analyses from 4 separate FTOC experiments (representing 7 mutant and 8 wild-type thymuses), there is a delay in progression ofHoxa-9−/− thymocytes through the ISP stage and significant reductions in the numbers of more mature DP and SP cells.

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