American Society of Hematology

Fig. 6.

$Fig. 6. Multiple defects in the TN check point of T-cell development in Hoxa-9−/− thymuses. Day-15.5 or day-16.5 fetal thymocytes were subjected to FACS analysis using the specific antibodies indicated in each panel. (A) Intracellular CD3 expression. The percentage of mutant thymocytes expressing intracellular CD3 is markedly reduced. (B) Intracellular TCRβ expression. Approximately one fourth of the normal fetal thymocytes have successfully rearranged their TCRβ chain by day 16.5, whereas the extent of TCRβ rearrangement in theHoxa-9−/− T cells is diminished by fourfold. (C) IL-7R expression. The percentage of IL-7R+ thymocytes in Hoxa-9−/− thymuses is significantly decreased as is the mean fluorescence intensity. (D) E-cadherin expression on fetal thymocytes. The fraction ofHoxa-9−/− thymocytes expressing high levels of E-cadherin is diminished by half. All analyses were repeated with at least 3 separate mutant and control animals.$

Multiple defects in the TN check point of T-cell development in Hoxa-9^−/− thymuses. Day-15.5 or day-16.5 fetal thymocytes were subjected to FACS analysis using the specific antibodies indicated in each panel. (A) Intracellular CD3 expression. The percentage of mutant thymocytes expressing intracellular CD3 is markedly reduced. (B) Intracellular TCRβ expression. Approximately one fourth of the normal fetal thymocytes have successfully rearranged their TCRβ chain by day 16.5, whereas the extent of TCRβ rearrangement in theHoxa-9^−/− T cells is diminished by fourfold. (C) IL-7R expression. The percentage of IL-7R⁺ thymocytes in Hoxa-9^−/− thymuses is significantly decreased as is the mean fluorescence intensity. (D) E-cadherin expression on fetal thymocytes. The fraction ofHoxa-9^−/− thymocytes expressing high levels of E-cadherin is diminished by half. All analyses were repeated with at least 3 separate mutant and control animals.

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