ODN Uptake. (A) ODN is found in clathrin coated pits indicative of receptor-mediated endocytosis. (B and C) ODN can be found in lysosomal (clear vesicles) or endosomal compartments (vesicles filled with darker material); however, some ODN is free and (D) crosses the nuclear membrane to presumably form hybrids with target mRNA. (E) Control panel labeled with Biotin alone. Oligodeoxynucleotides have been decorated with gold beads that appear has black dots in the photomicrograph. Their location is pointed to by black arrows. (Reproduced from The Journal of Clinical Investigation, 1995, vol 95, p 1814 by copyright permission of The American Society for Clinical Investigation.147)