Effects of SDF-1α on the production of megakaryocytes from CD34⁺ cells. CD34⁺cells were isolated from bone marrow by immunoadsorption using the CellPro Ceprate LC system as described in Materials and Methods. The cells were cultured in a liquid culture using serum-free medium with growth factors as indicated. After 10 days of culture, megakaryocyte production was assessed by immunofluorescence staining using an anti-CD61 MoAb and cell counting. Results represent the mean ± SD of three separate experiments. * shows statistical significance compared with the control without SDF-1α and is assessed as P < .05.