Fig. 3.
Effect of GM-CSF deprivation on expression of MDM2 and p53 proteins, as well as p53 protein binding to MDM2. Total cell lysates were obtained from TF-1 AML cells (5 × 106 viable cells/sample) at 0, 2, 4, 8, and 16 hours after GM-CSF deprivation and immunoprecipitated with anti-MDM2 or anti-p53 MoAbs, and immunoblotted with the same MoAb. In addition, the same cell lysates were immunoprecipitated with anti-MDM2 MoAb and immunoblotted with anti-p53 MoAb. IP and WB with antiactin MoAb confirmed equal protein loading.
