Status of p53 in TF-1 AML cells. Amplicons from exon IV to exon IX of the p53 gene were obtained by PCR from genomic DNA of TF-1 AML cells, and the DNA was directly sequenced using appropriate sequencing primers. (A) The upper panel shows the wtp53 sequence of TF-1 cells, and the lower panel shows a 1 bp deletion at codon 251 in exon VI (ATC∅︀AC). (B) TF-1 cells were cultured for 2 days in fresh media with GM-CSF (1 ng/mL). After exposure to γ-irradiation (mock or 2.0 Gy), cells were cultured for an additional 2 hours or 16 hours . Total cell lysates from 5 × 106 viable cells/sample were immunoprecipitated with anti-p53 MoAb after 2 hours of culture and immunoblotted with horseradish peroxidase conjugated p53 MoAb. Similarly, cell lysates were immunoprecipitated with anti-p21 MoAb after 16 hours of culture and immunoblotted with anti-p21 MoAb. Equal protein loading was confirmed by IP and WB for actin. {/ANNT;4224n;;2112n;2112n}A {/ANNT;4224n;;2112n;124256n}B
