Fig. 6.
Percentage of marking calculated by semiquantitative PCR originating from both the nonexpanded and expanded population of marked cells posttransplant for all 8 animals. For each animal, the upper panel represents the percentage of marking originating from transduced but nonexpanded cells (NE), while the lower panel represents the percentage of marking from the expanded cells (EXP). Marking was assessed in PB mononuclear cells (PB monos), PB granulocytes (PB Grans), and BM mononuclear cells (BM monos) at 2 weeks, 2 months, 3 months, 6 months, 9 months, and 12 months. In each panel, the ratio of marking from the nonexpanded cells to the marking from the expanded cells is given for each fraction analyzed at each time point in the upper portion of each panel. A ratio greater than 1 signifies higher marking from the nonexpanded cells, and ∞ denotes no detectable marking from the expanded cells, at least a 3 log difference given the PCR sensitivity.