Southern blot analysis of genetic marking in animal RC402. Ten micrograms of genomic DNA was digested with Kpn I, which cuts within the viral LTRs. In this animal, the nonexpanded fraction was transduced with LNL6 and the expanded fraction with G1Na in the presence of IL-3, IL-6, SCF, Flt-3 ligand, and an autologous stromal monolayer. PB mononuclear cells (PB MNCs) and BM mononuclear cells (BM MNCs) were analyzed, along with positive control dilutions of G1Na producer cell line DNA mixed with normal rhesus PB DNA at the percentages shown. The band present in these samples (3.0 kb) match the predicted size for LNL6 (3.0 kb).