Globin expression analysis of EBHX-derived colonies. Colonies were grown in the indicated cytokines for 7 days and then picked and analyzed for βH1 and βmajor expression by Poly(A) PCR. The analysis of three individual colonies is shown for each set of conditions. EBHX11-15 is a subclone of EBHX11. Control Eprepresents primitive erythroid colonies grown from day 6 EB precursors, control Ed are definitive erythroid colonies generated from fetal liver precursors, and control M are macrophage colonies grown from day 6 EB-derived precursors. N represents PCR reagents with no cells added. Expression of the ribosomal L32 gene was used as an indication of the amount of material in each lane. Cytokines were used at the concentrations indicated in the legend to Fig 3.
