Fig. 6.
Activation of human factor X by F.IX derivatives. The hydrolysis of chromogenic substrate S-2222 (0.4 mmol/L) by factor Xa (F.Xa) generated with 250 nmol/L of F.IX (▾), HNE-treated F.IX (○), or F.XIa-treated F.IX (•) is shown after using a SpectroMax 250 Plate Reader to record absorbance changes at 405 nm.