Induction of cytokines in MS-10 cells by the coculture with nonadherent BM cells. Nonadherent BM cells were cocultured with MS-10, and the expression of cytokines was analyzed by quantitative RT-PCR, MS-10 alone (lane 2); MS-10 cocultured with nonadherent BM cells for 48 hours (lane 3); nonadherent BM cells (lane 4); nonadherent BM cells cocultured with MS-10 for 48 hours (lane 5). Lane 1 is a positive control.