Induction of IL-11 production in MS-10 cells by the coculture with nonadherent BM cells. MS-10 cells were cocultured with nonadherent BM cells for 6, 12, 24, and 48 hours. Expression of the genes for IL-1α and IL-11 in MS-10 cells was analyzed by quantitative RT-PCR, MS-10 alone (lane 2); MS-10 cocultured with nonadherent BM cells for 6 hours (lane 3); 12 hours (lane 4); 24 hours (lane 5); or 48 hours (lane 6); nonadherent BM cells (lane 7). Lane 1 is a positive control.