Detection of IL-11 molecules in conditioned media of the coculture of nonaderent BM cells and MS-10. MS-10 cells were cocultured with nonadherent BM cells for 6, 12, 24, 48, and 72 hours. IL-11 molecules were analyzed by immunochemistry. Negative controls (lane 1, conditioned medium of MS-10 alone and lane 8, 10% FCS in α-medium). Positive control (lane 2, conditioned medium of HB-1 cultured on MS-10). Conditioned medium of MS-10 cocultured with nonadherent BM cells for 6 hours (lane 3); 12 hours (lane 4); 24 hours (lane 5); 48 hours (lane 6); and 72 hours (lane 7). M lane 1 is molecular-weight marker.