Fig. 1.
Fig. 1. Overexpression of E2A-HLF in M1p53tsvalcells. (A) Western blot analysis of 10 μg protein/lane blotted with HLF antisera showing variable levels of E2A-HLF expression in clones 7, 24, and 25 compared with a lack of expression in parental cells (P). (B) Nuclear lysates prepared from E2A-HLF clones 7, 24, and 25 and studied by EMSA using a 32P-labeled oligonucleotide probe containing the HLF consensus binding sequence and supershifted with an E2A antibody. Supershifted complexes containing E2A-HLF were stable at both 0 hours (mutant conformation of p53 at 37°C) and 14 hours after a shift in temperature (wild-type conformation of p53 at 32°C).

Overexpression of E2A-HLF in M1p53tsvalcells. (A) Western blot analysis of 10 μg protein/lane blotted with HLF antisera showing variable levels of E2A-HLF expression in clones 7, 24, and 25 compared with a lack of expression in parental cells (P). (B) Nuclear lysates prepared from E2A-HLF clones 7, 24, and 25 and studied by EMSA using a 32P-labeled oligonucleotide probe containing the HLF consensus binding sequence and supershifted with an E2A antibody. Supershifted complexes containing E2A-HLF were stable at both 0 hours (mutant conformation of p53 at 37°C) and 14 hours after a shift in temperature (wild-type conformation of p53 at 32°C).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal