Fig. 5.
Fig. 5. M1p53tsval cell clones expressing human NFIL3. (A) Northern analysis of total RNA (20 μg/lane) prepared from 2 NFIL3-expressing clones (top panel) and probed with a human NFIL3 cDNA probe. Note the stability of the LTR-driven NFIL3 mRNA (solid arrowhead) in the absence of IL-6 at 32°C, the permissive temperature for p53. Rehybridization of the Northern blot with a mouseNfil3 probe demonstrated downregulation of the endogenousNfil3 mRNA (middle panel, open arrowhead) due to p53. Results for the Gapdh RNA loading control are also shown (bottom panel; ○). (B) EMSA results showing NFIL3 binding activity of clones 2, 3, 4, 7, and 8 (lanes 3 through 12) versus control cells (lanes 1 and 2) at 37°C and 14 hours after shift to 32°C.

M1p53tsval cell clones expressing human NFIL3. (A) Northern analysis of total RNA (20 μg/lane) prepared from 2 NFIL3-expressing clones (top panel) and probed with a human NFIL3 cDNA probe. Note the stability of the LTR-driven NFIL3 mRNA (solid arrowhead) in the absence of IL-6 at 32°C, the permissive temperature for p53. Rehybridization of the Northern blot with a mouseNfil3 probe demonstrated downregulation of the endogenousNfil3 mRNA (middle panel, open arrowhead) due to p53. Results for the Gapdh RNA loading control are also shown (bottom panel; ○). (B) EMSA results showing NFIL3 binding activity of clones 2, 3, 4, 7, and 8 (lanes 3 through 12) versus control cells (lanes 1 and 2) at 37°C and 14 hours after shift to 32°C.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal