Fig. 2.
An agarose gel showing the calibration of sensitivity for the seminested CDR3 PCR amplification. The amplification product has a length of approximately 350 bp. Cells from the malignant lymph node (which contained 80% tumor cells) were serially diluted into normal spleen cells down to 1 abnormal cell in 107 splenocytes. Three separate dilutions of 1 in 107 were tested. Normal spleen and no DNA controls are included. At the bottom are shown the same cDNA preparations amplified with beta-2 microglobulin primers as a control for quality of the cDNA and for gel loading.
