Cell-cycle analysis of nonadherent and cobblestone area–forming cells generated after the coculture of mobilized PB CD34⁺ cells with stromal cells. Mobilized PB CD34⁺ cells were cultured on an MS-5 monolayer. On day 4, nonadherent and adherent cells were discarded. On day 6, when nonadherent cells, adherent cells, and cobblestone area–forming cells appeared, the three populations of the cells were collected and stained with anti-CD34 MoAb. CD34⁺ cells were analyzed as to cell-cycle status. The adherent cells were too few for the analysis. The percentages of CD34⁺ cells in S + G₂/M phase are presented. *P < .0001 by paired t-test.