Fig. 8.
Effect of CD23 ligation and L-NMMA on the apoptosis of B-CLL cells. Purified leukemic B cells from a freshly collected B-CLL sample were labeled with FITC-annexin V, either at day 0 (A), or after 2-day culture with medium alone (B), or with anti-CD23 MoAb (clone 135; 10 μg/mL) (C), or with anti-CD23 MoAb (10 μg/mL) and L-NMMA (1 mmol/L) (D). An FITC-labeled goat anti-rabbit Ig F(ab′)2 was used as a control of nonspecific binding. Dot plots (SSC v FSC) and percentage of annexin V+ cells were analyzed by the Procyt software. The data are from one representative experiment out of three.
