Fig. 9.
Overexpression of Bcl-3 and p50 or p52 activates ac-myb κB-TATA-luciferase reporter plasmid. A total of 0.5 μg of plasmids expressing Bcl-3, p50, or p52 was cotransfected with 1.0 μg of c-myb κB-TATA-luciferase reporter plasmid into TF-1 cells separately or in combination. The pGL2 basic vector was used as negative control. The total amount of transfected DNA (2 μg) was kept constant by adding appropriate amounts of expression vector without insert. At 48 hours after transfection, cells were collected for luciferase assay. Results are expressed as the mean ± SEM (×103 cpm). Three experiments were performed. *A significant increase above the c-myb κB-TATA reporter plasmid (P < .05).