Fig. 2.
The multiple forms of SHIP can be generated from a single cDNA and are all long lived in vivo. (A) An anti-HA Western blot of anti-N + anti-M (left panel) or anti-C immunoprecipitates (right panel) from IL-3–stimulated DA-ER cells expressing HA-tagged SHIP. (B) An autoradiogram of anti-HA immunoprecipitates from 35S-methionine pulse-labeled HA-SHIP–expressing DA-ER cells taken at 0 (−), 1, 3, 10, and 21 hours after the 30-minute labeling period. Similar results were obtained in 3 separate experiments.
